 หัวเรื่อง:การเพาะเซลล์ BHK21C13 แบบซัสเปนชั่นด้วยน้ำยาเลี้ยงเซลล์ที่มีซีรั่ม 5๔ เพื่อการผลิตไวรัสและวัคซีนโรคปากและเท้าเปื่อย ผู้เขียน: Payont Sinsuwonkwat, Cherngchai Chumtarusmi สื่อสิ่งพิมพ์:pdf AbstractThese experiments were to decrease the amount of PEG treated serum (Bovine serum collecting from slaughter house and treated with 8% PEG 6000) in the medium without changing the character of cultured cells in order to produce high yield of FMD virus and good quality of FMD vaccine. The BHK21C13 suspension cell cultured in the medium containing 5% poly ethylene glycol (PEG) treated serum for 3 days at 37oC. pH 7.2 The cells were subcultured in the same medium and condition until the 7th passage. The 7th passage cells were inoculated by type 0 cattle 0 (OBKK/S1) FMD virus by cell sedimentation method. The inoculated cells were culture in the medium containing 1% PEG treated serum at 37oC. pH 7.4 and harvested at 18 hours for preparing the vaccines. The results showed that the BHK21C13 suspension cells could grow in the medium containing 5% PEG treated serum, and could produce high yield of FMD virus and good quality of FMD vaccine. |